Uv-spectophotometric Method Development and Validation of Synthesized 7-hydroxy-4-methyl Coumarin.
Year: 2024
Type: article
Abstract: Objectives: The objectives of present workwastoo develop and standardize UV-Spectrophotometric method for estimation of 7-Hydroxy-4-Methyl Coumarin in marketed formulation. Materials and method: UV- Spectrophotometric method was developed using 70ml water: 30ml methanol as solvent. The developed method was standardized in terms of validation parameters such as simple, sensitive, precise, linear, accurate, robust, reproducible as per ICH Q2(R1) guidelines. For estimation of 7-Hydroxy-4-Methyl Coumarin in marketed formulation this newly developed method was successfully applied. Results: 7-Hydroxy-4-Methyl Coumarin exhibits λmax at 321nm in the Beer’s range 2 to 10μg/ml Beer’s law was obeyed. The limit of detection was found to be 0.84μg/ml and limit of quantification was found to be 2.54μg/ml. Recovery of 7-Hydroxy-4-Methyl Coumarin in marketed formulation was obtained in the range of 75-100%. All the precision range less than 2%. Conclusion:The method was found to be simple, accurate, environment friendly, reproducible and marketed API of 7-Hydroxy-4-Methyl Coumarin can be estimated.
Author: Tabassum M. Inamdar*, Udayakumar Bolmal, Vani Magdum, Prema kumbar, Biradar Kiran, Sakib Nazirahmed Desai, Ankusha Nanamutti
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6949067640
FWCI:
Cites:
Cited by:
Related to: