Work

mRNA-LNP Encapsulation

Year: 2024

Type: article

Abstract: LNPs are liposome-like structures, engineered for encapsulating a broad variety of nucleic acids (RNA, mRNA, siRNA, gRNA, circRNA and DNA) and APIs; LNPs consist in an aqueous core surrounded by a lipidic shell based on a combination of 4 families of chemicals: an ionizable cationic lipid, a polyethylene-glycol (PEG) lipid, cholesterol, and a helper phospholipid. The ionizable cationic lipids are used to sequester the nucleic acids through charge absorption. The PEG lipids are incorporated to increase the circulatory half-life in the body. Cholesterol is a structural "helper" lipid that improves efficacy possibly by promoting membrane fusion and promoting endosomal escape. Similarly, the helper phospholipids are also commonly used in LNP formulation to promote cell binding.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.14221238

Author: Brady, Marcia

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W6949480528

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