Allosteric regulation of the Golgi-localized PPM1H phosphatase by Rab GTPases modulates LRRK2 substrate dephosphorylation in Parkinson's disease
Year: 2025
Type: article
Abstract: Figure 2.zipContains all raw data and analysis related to Microscale Thermophoresis (MST) binding experiments between wild-type (WT) PPM1H or L66R PPM1H and Rab8A, Rab10, and Rab12. This includes: CSV files of raw MST measurements GraphPad Prism files used to generate binding curves Figure 3.zipContains MST data for WT and L66R PPM1H binding to phosphorylated Rab8A (pRab8A): Raw data in CSV format Corresponding Prism files for curve fitting and analysis Figure 4.zipContains data for co-flotation assays of WT and L66R PPM1H with Rab8A, including: Primary immunoblot images (TIFF format) Annotated blot images (PDF) Gel quantification data (CSV) GraphPad Prism analysis files Figure 5.zipCo-flotation data for WT and L66R PPM1H with Rab10: Primary and annotated blot images Quantification (CSV) Prism analysis files Figure 6.zipCo-flotation assay data for WT and L66R PPM1H with Rab12: Raw immunoblots (TIFF), annotated PDFs Quantification and Prism analysis files Figure 7.zipData for the inhibition of PPM1H activity by unphosphorylated Rab8A: Immunoblot data (TIFF and annotated PDF) Gel quantification (CSV) Prism files analyzing the inhibition curve Figure 8.zipContains data for D37 PPM1H experiments: Co-flotation with Rab8A (TIFF and annotated immunoblots, CSV quantitation, Prism analysis) MST binding experiments of D37 PPM1H with Rab8A and Rab10 (CSV files, Prism files) Figure S2. zip Contains the raw file for the phostag gel and the corresponding quantitaion in CSV file. Analysis in Prism.
Author: Adhikari, Ayan
Institution: Stanford Medicine
Language: English
Subfield: Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W6949509780
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