Hortiboletus bubalinus L. Albert & Dima
Year: 2025
Type: article
Abstract: Hortiboletus bubalinus (Oolbekk. & Duin) L. Albert & Dima, Index Fungorum 251: 1 (2015) Figs 8, 9, 10 ≡ Boletus bubalinus Oolbekk. & Duin, in Oolbekkink, Persoonia 14 (3): 367 (1991) ≡ Xerocomellus bubalinus (Oolbekk. & Duin) Mikšík, Index Fungorum 182: 1 (2014) ≡ Xerocomus bubalinus (Oolbekk. & Duin) Redeuilh, Docums Mycol. 23 (no. 89): 62 (1993) ? = Xerocomus erubescens Cadiñanos & J. A. Muñoz, Belarra 9: 61 (1992) ? – ‘ Boletus populinus ’ Oolbekk. & Duin (1988), Coolia 31 (1): 11, nom. illeg., nom. prov., Art. 53.1 (Shenzhen), non B. populinus Schumach., Enum. Pl. 2: 384 (1803), q. e. Oxyporus populinus (Schumach.) Donk Holotype examined here. NETHERLANDS • North Holland: Aerdenhout, A. W. dunes, near Ezelenvlak, on sandy soil, under Pinus and Populus, 14. 10. 1983, leg. G. Oolbekkink & W. van Duin, L 0053449 (G. Oolbekkink & W. van Duin 145 – collector’s number), GenBank: ITS – PV 094766 and PV 094767. Description. Basidiomes pileate-stipitate, xerocomoid, epigeal, small to medium-small sized. Ontogenetic development gymnocarpic. Pileus (2.9 –) 3.8–10.3 (– 13.5) cm broad, firstly hemispherical then persistently convex and finally broadly pulvinate-flattened, sometimes slightly depressed at centre, regularly to hardly unevenly shaped by shallow depressions, margin even to faintly wavy-lobed, initially involute then curved downwards or rarely completely plane, not or only a little extending beyond the tubes (up to 1 mm); surface matt, dry, but slightly greasy in aged basidiomes with moist weather, markedly wrinkled and finely pruinose in the early stage of development then tomentose with age, usually not cracked or occasionally areolate towards the peripheral zone; somewhat variable in colour, at first, dark garnet red or dark reddish-brown, but typically paler towards margin (pale orange to yellowish-orange), often later gradually fading to drab tinges and becoming orange ochraceous, sometimes with scattered pinkish hues, then evenly pale ochraceous brown, chamois olive to dull ochraceous buff, often retaining small and dispersed pale-reddish to ochraceous-orange zones (especially in the depressions) and with a thin, reddish-pink marginal band; aged basidiomes can completely lack reddish tones, although some basidiomes retain bright reddish tints with age; unchanging on handling or touching or when injured; subpellis layer pale pink to whitish buff. Tubes up to 12 mm long, at first, thin in side view then increasingly wider with age and shorter than the thickness of the pileus context, easily separable from the context above, adnate, but soon depressed around the stipe apex and shortly decurrent with a tooth, bright yellow to olive yellow then olive brown, bluing on cutting. Pores initially forming a concave or ascending surface, later flattening, at first very small then gradually wider with age (up to 2.0 mm in diameter), compound, labyrinthine becoming angular at maturity, concolourous with the tubes; strongly bluing on bruising and later becoming dirty brownish. Stipe (3.1 –) 4.3–7.3 (– 8.3) × 1.1–2.7 (– 3.2) cm, as long as the pileus diameter at maturity, otherwise slightly to moderately shorter or longer, central to slightly off-centre, solid, firm, dry, straight or faintly curved, at first ventricose or clavate although tapering at the very base, later more or less cylindrical, sometimes remaining inflated downwards or progressively tapering from apex down to the base, not or slightly rooting; surface smooth or with fine punctuations from apex down to the middle part, longitudinally fibrillose with age, without reticulum; usually bright yellow or somewhat reddish at the very apex, flesh pink, reddish-pink to wine red elsewhere, rarely yellow to pale yellow throughout, usually progressively and sometimes completely losing reddish tones with age similarly to the pileus surface and eventually becoming dull buff; yellow ochraceous at the very base, sometimes with brownish-olive hues, occasionally with purplish patches; unchanging or faintly bluing on pressure and finally turning brownish; basal mycelium yellow. Context firm and tough when young, later soft textured and eventually flabby in the pileus (up to 2.3 cm thick in the central zone, up to 1.5 mm thick halfway to margin and gradually becoming thinner towards the edge), fibrous in the stipe, pinkish-red in the pileus in very young to young basidiomes, but with a thin straw yellow to whitish layer above the tubes, becoming pale flesh pink at maturity and eventually fading to dull white in aged basidiomes or on drying; pale yellowish in the stipe, but progressively duller downwards, dirty yellow ochraceous at the very base and, as a rule, devoid of carrot-orange to orange-red dots, which can rarely be present; quickly bruising from light green-blue to blue on exposure, particularly in the pileus-stipe connection zone, above the tubes and in the peripheral layers of the stipe; after a few minutes, the bluing extends quite intensely to the lower part of the stipe (particularly in young basidiomes) and later becomes drab yellowish-olive, unchanging in the pinkish-red zone underneath the pileus surface; the oxidation phenomenon is less intense in old fruiting bodies and can be absent in very old basidiomes; context pinkish in the areas eaten by slugs and brownish where eroded by maggots; subhymenophoral layer yellowish-white; exsiccate brownish and reddish tones completely disappear during drying process. Spore print olive brown. Odour strong, pleasant, fruit-like but with an “ iron ” component. Taste mild. Macrochemical spot-test reactions: Melzer’s reagent: a weak fleeting-amyloid reaction on the hymenophoral trama and on the stipe and pileus context of fresh and dry basidiomes; 20 % KOH: pale to bright orange on hymenophore and context, reddish-brown on pileus and stipe surface; 25 % NH 4 OH: none; 10 % FeSO 4: none; 10 % H 2 SO 4: none on context, orange on pileus and stipe surface and hymenophore. Basidiospores [1020 / 35 / 35] (10.7 –) 11.7 ± 0.6 (– 13.3) × (4.3 –) 4.8 ± 0.2 (– 5.3) μm, Q = (2.26 –) 2.46 ± 0.10 (– 2.70), Vm = 140 ± 20 μm 3, sometimes abnormally large – up to 19.0 × 7.5 μm, inequilateral, fusiform to ellipsoid-fusiform in side view, narrowly or moderately ellipsoid to amygdaliform in face view, smooth, apex rounded, with a short apiculus, usually with a distinct suprahilar depression and with a slightly pronounced adaxial swelling, moderately to decidedly thick-walled (up to 1 μm), straw yellow to bright yellow-coloured in water and 5 % KOH, having one, two or three large oil droplets when mature, rarely pluri-guttulate, inamyloid and with an orthochromatic reaction. Basidia [101 / 7 / 7] (27.4 –) 36.1 ± 4.7 (– 44.2) × (8.8 –) 11.4 ± 1.4 (– 13.0) μm, subclavate to clavate, moderately thick-walled (0.5–0.7 μm), predominantly 4 - spored, sterigmata (1.5 –) 3.2 ± 0.8 (– 4.5) μm, hyaline to pale yellowish and containing straw-yellow oil guttules in water and 5 % KOH; basidioles subcylindrical to faintly clavate, similar in size to basidia. Cheilocystidia [20 / 3 / 3] (45.4 –) 56.7 ± 10.9 (– 72.3) × (6.9 –) 8.6 ± 1.8 (– 11.5) μm, relatively common, moderately slender, projecting straight to sometimes flexuous, subcylindrical-fusiform, fusiform to ventricose-fusiform, with rounded tip, smooth, moderately thick-walled (0.5–1.0 μm), hyaline to pale yellowish in water and 5 % KOH, without epiparietal encrustations. Pleurocystidia [38 / 3 / 3] (40.9 –) 56.6 ± 8.0 (– 77.9) × (7.9 –) 11.8 ± 2.3 (– 16.5) μm, infrequent, shape, size, colour and chemical reactions similar to cheilocystidia. Pseudocystidia not observed. Hymenophoral trama bilaterally divergent intermediate between the “ Phylloporus - type ” and the “ Boletus - type ”, but quite closer to the former, with moderately divergent and tightly arranged, non-gelatinous hyphae (lateral strata hyphae in transversal section touching or almost touching each other, 0–4 (– 5) μm apart), hyaline to very pale yellowish in water and 5 % KOH, inamyloid in Melzer’s; mediostratum axially arranged, consisting of a tightly adpressed, non-gelatinous bundle of hyphae; in Congo red, the mediostratum is concolourous with or slightly darker than the lateral strata. Pileipellis a physalo-palisadoderm consisting of erect subparallel to parallel chains of short, stout, septate hyphae; terminal elements [1023 / 29 / 28] (17.5 –) 28.7 ± 8.1 (– 58.5) × (7.1 –) 13.2 ± 3.1 (– 20.3) μm, Q = (1.63 –) 2.31 ± 0.66 (– 4.84), versiform, usually more or less swollen, mainly bullet-shaped or acorn-shaped, less frequently broadly cylindrical, ventricose to ovoid or cystidioid, but also globose or subglobose, sometimes mucronate, more rarely peanut-shaped, conical or utriform, occasionally lanceolate or filamentous and sinuous and then particularly long (up to 86 μm long), often bifurcate, apex rounded-obtuse or pointed, moderately thick-walled (up to 1 μm), hyaline to pale yellow in water and 5 % KOH; subterminal elements usually inflated, swollen to vesiculose and broader than terminal ones (up to 38 μm broad), often branched; pileipellis elements moderately to strongly ornamented by granular or zebra-like epiparietal encrustations, more evident and pronounced on subpellis hyphae, but also present on terminal cells surface, although less outlined, brownish in 5 % KOH. Stipitipellis a layer of slender, parallel to subparallel and longitudinally running, smooth-walled, adpressed hyphae, 5–14 μm broad, hyaline to pale yellowish in water and 5 % KOH; the stipe apex is covered by a well-developed caulohymenial layer consisting of sterile caulobasidioles, very sparse, predominantly 2 - and 4 - spored, fertile caulobasidia and scattered projecting caulocystidia, similar in shape and size to the hymenial cystidia; elsewhere, the stipe surface exhibits isolated clusters of cystidioid to clavate, sterile elements, 4–9 μm broad. Lateral stipe stratum under the caulohymenium only rarely present and obscurely differentiated from the s
Institutions: Royal Botanic Gardens, Kew, HUN-REN Szegedi Biológiai Kutatóközpont, Institute of Biochemistry, Hungarian Research Network, University of Haifa, Tula State Pedagogical University named after L.N. Tolstoy, James Hutton Institute, Komarov Botanical Institute of the Russian Academy of Sciences, Agricultural University of Athens, National Academy of Sciences of Ukraine, M.G. Kholodny Institute of Botany, University of Turin, Eötvös Loránd University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6949952471
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