Isolation and characterisation of stress tolerant divalent dependant lectin from insect pest of Corcyra cephalonica (Stainton) (Lepidoptera: Galleriidae) and its functional studies
Year: 2025
Type: article
Abstract: This study aims to isolate and identify a lectin from an agricultural insect pest of Corcyra cephalonica (CC). The lectin exhibited strong hemagglutination in sheep and goat blood, particularly A+ and B+, influenced by metal ions Ca2+ and Mn2+. Its molecular mass was determined to be 225 kDa, comprising subunits of 95, 68, and 62 kDa. The lectin displayed high thermal stability up to 100 °C and maintained hemagglutination activity at pH 12.0. MALDI-TOF analysis confirmed its identity as a lectin. Notably, it displayed significant antioxidant activity, achieving 93.98% inhibition against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 97.59% against 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) at concentrations of 0.5 and 0.05 mg/mL, respectively. This is the first report of Corcyra cephalonica lectin exhibiting such potent antioxidant properties, suggesting its potential as an ingredient in drug development for free radical-mediated inflammatory diseases and cancer since it has high biophysical stability.
Source: Figshare
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W6958934486
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