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A sensorised, microfluidic Ussing chamber manufactured by stereolithography and soft lithography techniques

Year: 2024

Type: article

Abstract: \nMotivated by the need for improved platforms in biomedical research, this study addresses challenges associated with traditional Ussing chamber systems, widely used in studying biological barriers like the epithelial barrier of the gut. These challenges include complexity, high sample volumes, and limited compatibility. By combining stereolithography and soft lithography techniques, a microfluidic Ussing chamber is manufactured, overcoming these limitations, and incorporating compatibility with microscopy. Validation through Trans-Epithelial Electrical Resistance (TEER) measurements confirms its efficacy in assessing ion permeability dynamics, utilizing Caco-2 cell monolayers. The study showcases the capability of the manufactured chamber for sensing impact of calcium on tight junctions.\n

Source: Springer Link (Chiba Institute of Technology)

DOI: 10.1051/epjconf/202430910007/pdf

Authors: Bastián Carnero, Yago Radziunas-Salinas, Bruno K. Rodiño-Janeiro, Patrícia M. Rodrigues, Alar Ainla, Miguel Xavier, Lorena Diéguez, María Teresa Flores-Arias

Institutions: Universidade de Santiago de Compostela, International Iberian Nanotechnology Laboratory

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W6959371253

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