Plasmid invasion assays [Dataset]
Year: 2025
Type: dataset
Abstract: Plasmid invasion of a susceptible population along time (x axis), expressed as transconjugants per donor (Y axis) A) Invasion dynamics of wild-type plasmids pKM101 (red dots) and R388 (black dots) obtained at two different donor: recipient ratios (1:100 and 1:10,000). Asterisks over t = 60, t = 90 and t = 120 minutes indicate statistically significant differences between plasmids pKM101 and R388 with p < 0.05 (*) B) Mobilization dynamics obtained with TRA genes from PKM101 (traN, red) and tra genes from R388 (traW, black) integrated in the chromosome of E. coli. The mobilizable plasmid contained the MOB region of the corresponding plasmid. Differences between plasmids were statistically significant with p < 0.01(***) for all time points analysed C) Differences in the dynamics of conjugation (black) and mobilization (red) in plasmid pKM101. Mobilization assays were perform using a vector containing mobN harboured by a strain that contained the traN genes inserted in the chromosome. Conjugation dynamics were obtained using the wild type pKM101 plasmid. Statistical differences were observed at t = 90 (* p < 0.05) and t = 120 (** p < 0.01) D) Comparison of plasmid R388 conjugation dynamics (black dots) vs R388-derived constructions. Black dots correspond to the wild-type plasmid transferred to a recipient strain that contains an empty pBAD expression vector. Green dots represent values obtained in conjugations where donors contained wild-type R388, and recipients harboured a pBAD::pifC construction, able to block secondary transmission. White dots correspond to the mobilization of a plasmid containing the mob region of plasmid R388 from a strain harbouring a chromosomal integration of the TraW genes. Black lines indicate comparisons between groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). When not explicitly indicated, the comparison between measurements was non statistically significant (n.s.).
Authors: Rodríguez-Grande, Jorge, Ortiz, Yelina, García-López, Daniel, Garcillán-Barcia, M. Pilar, Cruz, Fernando de la, Fernández-López, Raúl
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6960340984
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