Determination of Peroxidase Activity Using Stop Reagent
Year: 2024
Type: article
Abstract: A number of substances inhibiting the activity of horseradish peroxidase have been studied. The most effective "stop reagents" are hydrochloric acid and concentrated hydrogen peroxide solution. A method for determining the activity of plant peroxidases by sampling using ortho-phenylenediamine as a reducing substrate and concentrated hydrogen peroxide solution as a stop reagent has been optimized. Keywords: peroxidase, ABTS, spectrophotometry, photocolorimetry, ortho-phenylenediamine.
Authors: Kravchenko, E. M., Makurina, O. V., Doroshkevich, V. S.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W6968091480
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