HPLC Development Method and Validation
Year: 2024
Type: article
Abstract: Chromatography, while primarily a separation technique, is widely used in chemical analysis. High-performance liquid chromatography (HPLC) is a highly versatile technology that separates analytes by passing them through a column filled with micrometer-sized particles. Today, reversed-phase chromatography is the most extensively utilized separation technique in HPLC. This is due to the reversed-phase method's simplicity, versatility, and scope, which can handle compounds with a wide range of polarity and molecular mass.This article discusses tactics and difficulties for planning HPLC method development and validation. Method development frequently follows well-established processes, such as buffer selection, mobile phase selection, and column selection. The approach developed should be as basic as feasible, and the optimum strategy is therotical and empirical approach.
Author: Akash Shelake*, Mrs. Jaya D. kamble, Dr. Nilesh Chougule
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6968093187