Flabellascoma coffeae L. Lu & Karun. 2025, sp. nov.
Year: 2025
Type: other
Abstract: Flabellascoma coffeae L. Lu & Karun. sp. nov. Fig. 8 Etymology. The species epithet “ coffeae ” refers to the host plant genus “ Coffea ” from which the fungus was isolated. Diagnosis. Differs from F. fusiforme D. F. Bao, Z. L. Luo, K. D. Hyde & H. Y. Su, by an internal chamber at both ends of ascospores. Holotype. HKAS 137607. Description. Saprobic on decaying branch of C. arabica. Teleomorph: Ascomata 280–450 µm high × 200–280 µm diam. (x- = 356 × 230 µm, n = 20) (including neck), solitary, rarely clustered, immersed, visible as black, crest-like ostiolar neck on the substrate, globose to subglobose, uniloculate. Ostioles central, with a pore-like opening, periphysate. Peridium 15–30 µm wide (x- = 24 µm, n = 30), composed of several layers of brown, thick-walled cells of textura angularis. Hamathecium 1.5–3 µm wide (x- = 2.2 µm, n = 30), hyphae-like, septate, branched, pseudoparaphyses. Asci 60–100 × 10–15 µm (x- = 75 × 12 µm, n = 30), 8 - spored, bitunicate, fissitunicate, cylindrical to clavate, straight, with a short furcate sessile, apically rounded with a broad ocular chamber. Ascospores 20–25 × 5–7 µm (x- = 22 × 6 µm, n = 30), overlapping biseriate, fusiform, hyaline, 1 - septate, constricted at the septum, the upper cell slightly wider than the lower cell, guttulate, smooth-walled, with a narrow bipolar sheath. Sheath drawn-out at both ends, 4–7 µm long × 2–3 µm wide (x- = 5.6 × 2.5 µm, n = 30), with an internal chamber at both ends of ascospores. Anamorph: Not observed. Culture characteristics. Ascospores germinating on PDA within 24 h, colonies reached 1.5–2 cm in diameter after twenty days at 25 ° C, circular, flat to umbonate, fluffy, smooth, with entire margin, from above, grey, from below, dark grey at the centre, white at the edge. Materials examined. China, Yunnan Province, Baoshan, on a decaying branch of Coffea arabica (Rubiaceae) (24°9'N, 98°8'E, 1050 m alt.), 30 July 2022, LiLu, BS 1 - C 3 (HKAS 137607, holotype), isotype MHZU 23-0063, ex-type living culture KUNCC 24-18341 = KUNCC 24-18342, ex-isotype living culture ZHKUCC 23-0636 = ZHKUCC 23-0637; China, Yunnan Province, Dali, on a decaying branch of C. arabica, (26°09'N, 101°91'E, 1416.46 m alt.), 25 July 2022, LiLu, DL-C 41 (HKAS 137612, paratype), isoparatype MHZU 23-0062, ex-paratype living culture KUNCC 24-18343 = KUNCC 24-18344, ex-isoparatype living culture ZHKUCC 23-0634 = ZHKUCC 23-0635. Notes. The phylogenetic result, based on SSU, ITS, LSU, RPB 2 and TEF 1 - α sequence data, showed our new collection Flabellascoma coffeae is close to F. fusiforme (Fig. 9). Flabellascoma coffeae can be distinguished from F. fusiforme in having an internal chamber at both ends of ascospores (Fig. 8; Bao et al. (2019)). Flabellascoma coffeae also fits well with the morphological characteristics of Flabellascoma, such as immersed ascomata with crest-like ostiolar neck, cylindrical-clavate asci and fusiform, hyaline, 1 - septate ascospores with a narrow bipolar sheath (Fig. 8; Hashimoto et al. (2018)). Based on nucleotide comparisons, F. coffeae (ZHKUCC 23-0636) is different from F. fusiforme (MFLUCC 18-1584) by 19 / 500 bp (3.8 %, without gaps) of the ITS, 7 / 835 bp (0.8 %, without gaps) of the LSU and 22 / 839 bp (2.7 %, without gaps) of the TEF 1 - α. In addition, the PHI test results (Fig. 22 d) revealed no significant recombination relationships between F. coffeae and its phylogenetically related taxa. Therefore, we introduce F. coffeae as a new species from coffee in China, based on morphology and multigene phylogeny.
Authors: Lu, Li, Karunarathna, Samantha C., Rajeshkumar, Kunhiraman C., Elgorban, Abdallah M., Jayawardena, Ruvishika S., Hongsanan, Sinang, Suwannarach, Nakarin, Kumla, Jaturong, Xiong, Yin-Ru, Hyde, Kevin D., Han, Mei-Yan, Zheng, De-Ge, Li, Qiang, Dai, Dong-Qin, Tibpromma, Saowaluck
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6968620468
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