Work

Information propagation through catalytic enzyme-free templating of DNA dimerisation with weak product inhibition

Year: 2023

Type: article

Abstract: Information propagation by sequence-specific, template-catalyzed molecular assembly is the source of the biochemical complexity of living systems, allowing thousands of sequence-defined proteins to be produced from only 20 distinct building blocks. By contrast, exploitation of this powerful chemical motif is rare in non-biological contexts, particularly enzyme-free environments, with even the template-catalyzed formation of dimers being a significant challenge. The main obstacle is product inhibition: the tendency of products to bind to their templates more strongly than individual monomers, preventing effective catalytic templating of longer polymers. Here we present a rationally designed enzyme-free system in which a DNA template catalyzes, with weak competitive product inhibition, the production of sequence-specific DNA dimers. We demonstrate the selective templating of 9 different dimers with high specificity and catalytic turnover. Most importantly, our mechanism demonstrates a rational design principle for engineering information propagation by molecular templating of longer polymers.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.8256586

Authors: Cabello-Garcia, Javier, Mukherjee, Rakesh, Bae, Wooli, Stan, Guy-Bart, Ouldridge, Thomas

Institutions: University College London, Imperial College London, University of Surrey

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green