Work

Analysis of Glycoproteins for Biomarker Discovery

Year: 2013

Type: book-chapter

Abstract: Glycoproteins play an important role in cell signaling and cell-cell interaction. The alterations of glycoproteins are often relevant to progression of diseases, and these changed glycoproteins can be important biomarkers. The lectin-based glycoproteomic technology has extensively been used for high-throughput screening of potential glycoprotein biomarkers. Here we describe a multi-lectin affinity chromatography and label-free quantitative glycoproteomic approach for discovery of glycoprotein biomarkers relevant to differentiation of glioblastoma stem cells.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-360-2_10

Authors: Jintang He, Yashu Liu, Jing Wu, David M. Lubman

Institutions: University of Michigan, Michigan Medicine

Language: English

Topic: Glycosylation and Glycoproteins Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: U.S. Public Health Service, National Cancer Institute, National Institute of General Medical Sciences