An Alternative Cascade for the Selective Methylation of Catechols and Tetrahydroisoquinolines by O-Methyltransferases
Year: 2024
Type: article
Abstract: Methyltransferases are gaining traction as a method to achieve greener and regioselective methylations of a wide array of substrates. In this work, a halide methyltransferase biomethylation cascade was successfully adapted with the incorporation of a methionine adenosyltransferase to generate the S‐adenosyl‐L‐methionine (SAM) methylation cofactor in situ from less costly ATP and L‐methionine, instead of directly adding SAM to start the cascade. Furthermore, this cascade was applied to achieve novel catechol and tetrahydroisoquinoline methylations with the O‐methyltransferases RnCOMT, MxSafC and NpN4OMT with high conversions and regioselectivities. In addition, the cascade was successfully trialled with the less toxic methyl group donor, methyl tosylate.
Authors: Salinger, Matthew T, Castellano Garrido, Daniel, Lamming, Eleanor D, Ward, John M, Moody, Thomas S, Jeffries, Jack WE, Hailes, Helen C
Institution: University College London
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6979831433
FWCI:
Cites:
Cited by:
Related to: