Work

Effect of Different Extraction Methods on Vitamin B12 from Blue Green Algae, Spirulina Platensis

Year: 2015

Type: article

Abstract: In the present study different methods of extraction of vitamin B 12 from Spirulina are compared.Six different extraction procedures were carried out and their effects of on total vitamin B 12 and the form of vitamin B 12 are presented.The results of the extraction method using KCN for the total vitamin B 12 and aqueous extraction for identifying the true form of vitamin B 12 in Spirulina are reproducible.The use of cyanide converted all forms of vitamin B 12 to a stable cyanocobalamin.Aqueous extraction helped in identifying the true form of vitamin B 12 in the algae.Presences of vitamin B 12 in the sample were compared by HPLC, microbiological, chemiluminescence and MS/MS methods.The results showed that the method of extraction influences the content of vitamin B 12 .Cyanocobalamin, a stable form of vitamin B 12 was quantified in Spirulina using microbiological assay, chemiluminescence assay and gold nanoparticle based RNA aptamer analysis and found correlation among these methods.

Source: Pharmaceutica Analytica Acta

DOI: 10.4172/2153-2435.1000337

Author: Anantharajappa Kumudha

Institutions: Central Food Technological Research Institute, Council of Scientific and Industrial Research

Language: English

Topic: Folate and B Vitamins Research

Subfield: Nutrition and Dietetics

Field: Nursing

Domain: Health Sciences

SDG: Zero hunger

Open Access status: hybrid

Funders: Council of Scientific and Industrial Research, India, Central Food Technological Research Institute, Council of Scientific and Industrial Research