Year: 2020
Type: article
Abstract: This study aims to develop nanoparticles with different types of solid and cationic \nlipids and evaluate them as siRNA delivery systems. For this purpose, we prepared \na series of lipid nanoparticles by combining cationic lipids (DOTMA, stearylamine, \nDDAB, and Esterquat-1) with solid lipids [Precirol ATO 5 (P), Compritol 888 ATO \n(C), Cetyl palmitate 15 (CP15)] by a modified hot microemulsion method. The \ndeveloped cationic solid lipid nanoparticles (cSLNs) were characterized in terms of \nparticle size, size distribution, and zeta potential using a Malvern NanoZS \nInstrument. After determining the complexation ability of cSLNs with siRNA, the \nResazurin assay was conducted to evaluate and compare their cytotoxicity. The \ncSLNs which successfully bind siRNA and show low cytotoxicity were further \ninvestigated by in vitro cellular uptake (fluorescence microscopy) and gene silencing \n(Western Blot) studies. According to characterization measurements, all \nformulations had a small particle size (< 200 nm), narrow size distribution \n(polydispersity index, PDI< 0.5), and high positive zeta potential (> +25 mV). The \ncSLN with Esterquat-1 was eliminated from further studies due to the poor \ncomplexation capacity. The use of cSLN with stearylamine was found inappropriate \nbecause of its high toxicity on both DU145 and PC-3 prostate cancer cells. None of \nthese formulations showed effective gene silencing on DU145 cells. Only cSLN \nincluding P:C mixture with DDAB or DOTMA were uptaken by PC-3 cells and \nshowed silencing efficiency. This study shows the importance of the lipid type \nselection for siRNA delivery systems and how this changes the transfection \nefficiency of these systems.
Author: Oner-Bozkurt, Ezgi
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6981651018