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Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling

Year: 2022
Type: conference-abstract
Abstract: Natural Deep Eutectic Solvents (NADES) are new generation of ionic liquids, and promising solvents in green extraction. NADES are made by mixing two or more compounds, hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD), at various molar ratios.1,2 In this paper 28 different NADES are used as eco-friendly alternatives for extraction of phenolic acids, flavonoids and anthocyanins from chokeberry with the aim of extraction efficiency screening. Twelve different compounds were used for preparation of NADES, among them choline-chloride, proline, urea, glycerol, malic acid, tartaric acid, glucose, fructose, etc. Ultrasound extraction was applied as an eco-friendly and highly-efficient technique for isolation of phenolics and solid phase extraction (SPE) was used for purification of extracts and removal of NADES compounds. Different extraction parameters were optimized: solid/ liquid ratio, volume of added water and loading volume of extracts on SPE. Repeatability of extraction procedure was assessed. Chemical profiles of the extracts were obtained by applying high-performance thin-layer chromatography (HPTLC) and mobile phase was optimized for simultaneously separation of anthocyanins and other phenolic compounds. Extraction efficiency of NADES were compered to acidified methanol as conventional solvents and 1% citric acid in water (w/v) which is a generally recognized as safe (GRAS) solvent. Principal component analysis (PCA) was performed to determine the similarity/dissimilarity between studied NADES and identify most important compounds (bands) for separation between them. PCA illustrate that extraction greatly depends on the compositions of eutectic mixture and shows that NADES can be “designer solvents”.
Language: English
Field: Chemistry
Open Access status: green
OpenAlex ID: W6982099657
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