Year: 2014
Type: article
Abstract: Current cell-based assays for determining the functional properties of high-density lipoproteins (HDL) have limitations. We report here the development of a new, robust fluorometric cell-free biochemical assay that measures HDL lipid peroxidation (HDLox) based on the oxidation of the fluorochrome Amplex Red. HDLox correlated with previously validated cell-based (r = 0.47, p<0.001) and cell-free assays (r = 0.46, p<0.001). HDLox distinguished dysfunctional HDL in established animal models of atherosclerosis and Human Immunodeficiency Virus (HIV) patients. Using an immunoaffinity method for capturing HDL, we demonstrate the utility of this novel assay for measuring HDLox in a high throughput format. Furthermore, HDLox correlated significantly with measures of cardiovascular diseases including carotid intima media thickness (r = 0.35, p<0.01) and subendocardial viability ratio (r = -0.21, p = 0.05) and physiological parameters such as metabolic and anthropometric parameters (p<0.05). In conclusion, we report the development of a new fluorometric method that offers a reproducible and rapid means for determining HDL function/quality that is suitable for high throughput implementation.
Source: PLoS ONE
Institution: University of California, Los Angeles
Language: English
Subfield: Biochemistry
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,382
Funders: American Heart Association, University of Washington, National Institutes of Health, Center for AIDS Research, University of Washington, National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Advancing Translational Sciences, UCLA AIDS Institute, Center for AIDS Research, University of California Los Angeles
Awards: G1312273583, CVD and Metabolic Complications of HIV/AIDS Data Coordinating Center, G201326576, G2681470406, Prospective Evaluation of Antiviral Therapy and Cardiac Health, G307593493, Molecular profiling of pro-inflammatory HDL, UCLA Clinical and Translational Science Institute, Oxidized HDL in the intersection of HIV, immune activation and atherosclerosis, G4163366182, Family and Neighborhood Interventions to Reduce Heart Disease Risk in East L.A., G5331288106, G5535366820, Glycemic Load and Resistance Training on Endothelial Function and Inflammation, G6600838650, G6938178204, UCLA Center for AIDS Research (CFAR), G794169821, The role of PON2 and PON3 proteins in atherosclerosis, G9000090879
OpenAlex ID: W2059416535