Antibacterial electrospun poly(ε-caprolactone) nanofiber mats loaded with cefazolin and meropenem for wound healing
Year: 2024
Type: conference-abstract
Abstract: Nanofiber wound dressings represent a promising approach to wound care, offering an adaptable platform for promoting faster and more effective wound healing. The goal of this research was the development of poly(ε-caprolactone) (PCL) nanofiber mats with incorporated antibiotics cefazolin (CEF) and meropenem (MER) for wound healing treatments. Nanofiber mats based on PCL (8 wt.% PCL prepared in 2,2,2-trifluoroethanol (TFE)) with cefazolin or meropenem (10 % of the polymer's weight) were produced using the blend electrospinning method. For comparison, PCL nanofibers without antibiotics were prepared by dissolving PCL pellets in TFE. The electrospinning parameters were a flow rate of 2.7 ml/h for PCL/CEF and 3 ml/h for neat PCL and PCL/MER solutions. The distance from the collector was 10 cm, and the voltage was 29 kV for all solutions. In the processed samples, cefazolin and meropenem maintained their biological activity and did not react with the PCL. The drug release profiles showed that the PCL/CEF released about 60 % of the drug, while the PCL/MER released about 40 % after 48 hours of incubation in Mueller-Hinton broth. Both PCL/CEF and PCL/MER nanofiber mats showed similar growth delays of Gram-positive clinical strains (S. aureus and S. epidermidis) compared to neat PCL. However, PCL/MER nanofiber mats efficiently inhibited the growth of Gram-negative clinical strains (E. coli, Klebsiella, Enterobacter spp., and Acinetobacter spp.). The effective antibacterial action of PCL/MER nanofiber mats could minimize infection-related inflammation, reducing the risk of complications like delayed healing, sepsis, and chronic wound formation. The overall results of this study have shown that novel PCL nanofiber mats could be used as a relevant drug scaffold, keeping the wound infection-free and promoting faster and more effective tissue repair.
Authors: Radisavljević, Anđela, Stojkovska, Jasmina, Jančić, Ivan, Božić, Dragana, Perišić, Srđan, Stojanović, Dušica, Uskoković, Petar
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6989320879