Work

Interrogating the in vivo significance of selenium-based catalysis of GPX4 for mammalian development and survival

Year: 2018

Type: dissertation

Abstract: Glutathione peroxidase 4 (Gpx4) is a selenoprotein in mammals but Cys-containing homolog are found in other organisms. Its unique ability to reduce phospholipid hydroperoxides was reported to be necessary to suppress ferroptosis. To investigate the evolutionary advantage of selenolate- vs. thiolate-based catalysis in Gpx4, a transgenic mouse line was generated where the critical Sec of Gpx4 was replaced by Cys. Using this mouse line it was possible to show that the Cys variant of Gpx4 is sufficient for the survival of adult animals but Sec in Gpx4 is essential for embryogenesis and neurodevelopment in mice due to its inherent resistance towards peroxide-induced overoxidation.

Source: mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich)

Author: Ingold, Irina

Language: English

Topic: Selenium in Biological Systems

Subfield: Nutrition and Dietetics

Field: Nursing

Domain: Health Sciences

Open Access status: green

Funders: Università degli Studi di Padova, Karolinska Institutet, Rheinische Friedrich-Wilhelms-Universität Bonn

OpenAlex ID: W6991916412

Cites:

Cited by:

Related to: 10