[Protocol] Hi-C2B: optimized detection of chromosomal contacts within synchronized meiotic S. cerevisiae cells
Year: 2024
Type: article
Abstract: Hi-C, a genome-wide chromosome conformation capture assay, is a powerful tool used to study three-dimensional genome organization by converting physical pairwise interactions into counts of pairwise interactions. To study the many temporally regulated facets of meiotic recombination in S. cerevisiae, the Hi-C assay must be robust such that fine- and wide-scale comparisons between genetic datasets can be made. Here we describe an updated protocol for Hi-C (Hi-C2B) that generates reproducible libraries of interaction data with low noise and for a relatively low cost.
Source: Figshare
Authors: Ellie May Wright (5085611), Stephanie Schalbetter (11734226), Matt Neale (4461337)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6995992079
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