Year: 2017
Type: dissertation
Abstract: Lactic acid has many uses as food chemicals, cosmetics, medicines, and raw materials for biodegradable polymers.Lactic acid has been effectively produced by fermentation using various sugars, such as glucose, sucrose, and starch prepared from agricultural products such as sugarcane, corn, and potato.In this study, optimization of lactic acid production using banana peel and corncob as unutilized raw biomaterials for multiple parallel fermentation (MPF) via Leuconostoc mesenteroides NBRC 3832, Bacillus licheniformis YN15, and Aspergillus awamori NBRC 4388 has been investigated using the Taguchi method.At first, optimal conditions of D-lactic acid production from banana peel and corncob by MPF with L. mesenteroides and A. awamori have been determined.(1) D-Lactic acid production of 31.8 g/L was produced in the optimized medium containing 15% banana peel, 0.5% KH2PO4, 0.1% Tween 80, 0.05% MgSO4•7H2O, 0.05% NaCl, 1.5% yeast extract, and 0.2% diammonium hydrogen citrate at 30 ° C for 36 h.(2) D-Lactic acid production of 38.3 g/L was produced in the optimized medium containing 15% of corncob, 0.5% KH2PO4, 0.1% Tween 80, 0.05% MgSO4•7H2O, 0.1% NaCl, 1.0% yeast extract, and 0.4% diammonium hydrogen citrate at 30 ° C for 36 h.Next, optimal condition of Llactic acid production from banana peel by MPF using B. licheniformis and A. awamori has been determined.(3) L-Lactic acid production of 28.0 g/L was produced in the optimized medium containing 10% banana peel, 0.5% potassium dihydrogen phosphate, 0.05% Tween 80, 0.1% magnesium sulfate heptahydrate, 0.1% sodium chloride, 1.5% yeast extract, and 0.2% ammonium sulfate at 30 ° C for 36 h.Thus, both banana peel and corncob are unutilized potential resources for lactic acid production.The result indicates that optimization of MPF could constitute part of a potential industrial application of the currently unutilized biomass through environmental-friendly lactic acid fermentation.
DOI: 10.34382/00009981
Author: ELYA, Mufidah
Institution: Ritsumeikan University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6996637002