Year: 2020
Type: article
Abstract: The role of mRNA decay in dynamic control of gene expression has long been appreciated, where changes in mRNA stability in response to various cellular conditions can have widespread effects on steady-state mRNA levels.While mRNA stability is controlled in part by ribonucleoprotein composition, the primary codon sequence within the open reading frame has increasingly been recognized as an important determinant of stability in eukaryotes, particularly with regard to differential utilization and incorporation of codons by the translation machinery.The data presented in this dissertation expand upon previous studies linking differences in codon composition in the mRNA open reading frame to effects on translation and stability in budding yeast (S. cerevisiae).The principle aim of this research was to characterize the effects of open reading frame sequence on mRNA decay rates in mammalian cells.Using a combination of single-gene mRNA reporters and transcriptome-wide half-life determinations by metabolic labeling in
Author: Forrest, Megan E
Institution: Case Western Reserve University
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7005515494