An atypical active cell death process underlies the fungicidal activity of ciclopirox olamine against the yeastSaccharomyces cerevisiae
Year: 2007
Type: article
Abstract: Ciclopirox olamine (CPO), a fungicidal agent widely used in clinical practice, induced in Saccharomyces cerevisiae an active cell death (ACD) process characterized by changes in nuclear morphology and chromatin condensation associated with the appearance of a population in the sub-G(0)/G(1) cell cycle phase and an arrest delay in the G(2)/M phases. This ACD was associated neither with intracellular reactive oxygen species (ROS) signaling, as revealed by the use of different classes of ROS scavengers, nor with a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive phenotype. Furthermore, CPO-induced cell death seems to be dependent on unknown protease activity but independent of the apoptotic regulators Aif1p and Yca1p and of autophagic pathways involving Apg5p, Apg8p and Uth1p. Our results show that CPO triggers in S. cerevisiae an atypical nonapoptotic, nonautophagic ACD with as yet unknown regulators.
Source: FEMS Yeast Research
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Awards: APOPTOTIC PATHWAYS IN SACCHAROMYCES CEREVISIAE A MODEL IN APOPTOSIS RESEARCH, Via(s) apoptótica(s) em Saccharomyces cerevisiae: padrão proteómico e interacções com a metacaspase, Estudo multidisciplinar sobre infecções fúngicas invasivas em pacientes imunossuprimidos: Susceptibilidade do hospedeiro ao fungo versus resistência do fungo a fármacos
OpenAlex ID: W2100151314