DNA Photolyase: Molecular Machinery for Repair of UV-Induced Damage in DNA
Year: 2021
Type: conference-paper
Abstract: Damages in DNA structure are often caused by UV light, ionizing radiation, toxic substances and \nenvironmental pollution. To maintain genetic stability, cells protect themselves against these kinds \nof lesions. Moreover, the main DNA repair processes in prokaryotic and eukaryotic cells are quite \nsimilar. Photolyases repair the major DNA defects-cyclobutane pyrimidine dimers (CPD) and \n(6-4)-photoproducts. The enzyme contains two photoactive cofactors: folate-the photon antenna \nmolecule and catalytically active FADH-form. Photolyase repairs UV (200-300 nm) induced damage in DNA by splitting the ring of CPD dimer into pyrimidine monomers. If not repaired the CPD lesions are highly cytotoxic, mutagenic, and carcinogenic. In the present theoretical/computational study of photolyase from E. coli, continuum electrostatic and electron tunneling currents methods are employed to get a full insight into photoactivation and \nphotorepair mechanism of the enzyme and the structure-function interrelation. Protonation state \nof titratable residues, redox potentials of the conserved tryptophan triad, the energetics and kinetic \nreaction rates are calculated comparing well with available experimental data. The free energies of \nall potentially relevant enzyme states during the photoreactivation mechanism are evaluated. This presentation also addresses a several long-time controversial questions about the biological \nrelevance of unusual U-shape of FADH cofactor; hopping vs. super-exchange mechanism of the ET pathway; the shortest FADH- to CPD distance, after flipping out the CPD damage to the active site, in the CPD-protein complex or presence of radical FADH* state in the resting state of photolyase. The study could be relevant for other types of photolyases and cryptochromes, which all share the same structural features.
Authors: Popović, Dragan, Stuchebrukhov, Alexei
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7018697946