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Identification of Improved Signal Peptides for Heterologous Expression in Saccharomyces using a Screen that Exploits Gaussia Luciferase

Year: 2025

Type: article

Abstract: A high-throughput and sensitive screen for the improved expression of gene targets in Saccharomyces cerevisiae is described that is based upon the activity of the luciferase from Gaussia princeps. Using the Unspecific Peroxygenase (UPO) from Agrocybe aegerita (AaeUPO) as a model protein, improvements in expression, effected through error-prone PCR-based mutation within the signal peptide (SP) domain, can be detected using fusion of the target to Gaussia luciferase encoded downstream of the first folded domain of the AaeUPO protein and luminescent assay of expression supernatants. In this way, previously undiscovered mutations within the SP of AaeUPO that improve expression were revealed, and then applied to the expression of full-length AaeUPO in S. cerevisiae. The system was validated against control expression constructs that were well or poorly expressed and indicated a 13.9-fold improvement in expression for the best mutant over the wild-type SP sequence. It is envisaged that this protocol may be applied generally to the high-throughput detection of improved expression in S. cerevisiae for constructs that are engineered using directed evolution techniques.

Source: White Rose Research Online (University of Leeds, The University of Sheffield, University of York)

Authors: Grogan, Gideon James, Camboni, Ginevra, Cartwright, Jared

Institution: University of York

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7019725032

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