[Protocol] CC-seq: nucleotide-resolution mapping of Spo11 DNA double-strand breaks in S. cerevisiae cells
Year: 2024
Type: article
Abstract: During meiosis, Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5' ends on both sides of the break during this process. Such Spo11 "covalent complexes" are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronized Saccharomyces cerevisiae meiotic cells.
Source: Figshare
Authors: George Brown (7143290), William Gittens (4464400), Rachal M Allison (16167704), Antony Oliver (4463038), Matt Neale (4461337)
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7023918288
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