Genetic variability in β-tubulin-1 in benzimidazole resistant Haemonchus contortus from sheep in north-east Punjab, Pakistan.
Year: 2014
Type: article
Abstract: Benzimidazole is a synthetic anthelmintic against which nematode resistance especially in \nHaemonchus contortus, is emerging at a alarming speed. The mechanism of benzimidazole resistance appears to \ninvolve mutations in the gene encoding β-tubulin isotype 1 (β-tubulin-1). The present study was carried out to find out \nthe variation existing in β-tubulin-1 which is directly involved with drug binding capacity involving microtubules \npolymerization. DNA of adult nematode H. contortus was extracted, amplified and sequenced. Out of 50 worms \ninvestigated, 37 showed benzimidazole susceptible gene while 13 were resistant indicating single nucleotide mutation \nat amino acid 200 TTC/TAC. In addition, 12 worms showed several regions of consistent difference indicating single \nnucleotide polymorphism (SNPs) at various positions in coding region. It has been concluded that resistant alleles \nconferring anthelmintic resistance is prevalent in the local population of H. contortus of north-east Punjab, Pakistan.
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
Funder: University of Glasgow
OpenAlex ID: W1520965791