Work

Using Sar Methodology for Identification of Freshwater Macrophyte Allelochemicals With High Anti-cyanobacterial Effect Against Planktonic Cyanobacteria

Year: 2023

Type: article

Abstract: Controlling harmful cyanobacterial “blooms” through developing a new generation of algaecides based on allelochemical substances is a challenge facing modern aquatic ecology and biotechnology. The present article is devoted to the use of the SAR (Structure-Activity-Relationship) information technology to identify allelochemicals from aquatic macrophytes (floating-leaved Nuphar lutea (L.) Sm. and several species of submerged macrophytes: Ceratophyllum demersum L., Myriophyllum spicatum L., Elodea canadensis Michx, and species of the genus Potamogeton) effective against planktonic cyanobacteria. Detection and identification of compounds were performed using gas chromatography-mass spectrometry. The PASS (Prediction of Activity Spectra for Substances) computer program has been applied to predict biological activity spectra of the major components of macrophyte metabolomes and discover their ecological potential against cyanobacteria. A study of the biological activities of major low-molecular-weight organic compounds showed that monocarboxylic acids, gallic acid, cis‑6-octadecenoic acid, cis‑9-octadecenoic acid, palmitoleic acid, linolenic acid, and 9-cis‑12-cis-linoleic \nacid are the most \npromising compounds for the experimental verification and creation of nature-like \nalgaecides of a new \ngeneration. PASS predictions were successfully compared to the available information on the biological \nactivity of those compounds and confirmed experimentally. The present study shows that some organic \nacids signiﬁcantly inhibit the growth of Synechocystis aquatilis Sauvageau and Aphanizomenon flos-aquae \nRalfs ex Bornet and Flahault and can be used as algaecides for suppression of cyanobacteria. The \ninhibitory effect of the combined mixture of these allelochemicals is stronger than the effect of each \nindividual component, suggesting that there are various mechanisms of cyanobacterial growth inhibition

Source: SibFU Digital Repository (Siberian Federal University)