Year: 2024
Type: article
Abstract: Live Single-Cell Screening Based on Cell Encapsulation and Irradiation of Patterned Light Approx.800 words Single-cell screening has emerged as a powerful tool in life science, offering an effective method for detecting, isolating, and manipulating individual cells based on their specific characteristics.Traditional approaches involving manual identification of single cells from images were labor-intensive and time-consuming.Image-based cell sorting (IBCS) addresses these limitations by employing image processing tools for efficient identification and processing of single cells from large populations.This thesis presents two novel light irradiation approaches for singlecell screening.Chapter 2 details the development of an automated photopolymerization system for encapsulating suspended single cells in photo-cross-linkable hydrogel.The system utilizes an image processing algorithm to identify individual cells from cell groups in captured images.A Digital Micromirror Device (DMD) transfers generated polymerization patterns, allowing targeted irradiation of suspended single cells in gelatin methacryloyl (GelMA) for encapsulation.Three data transfer methods were developed and evaluated based on their encapsulation rates and processing times.Chapter 3 explores the potential of GelMA for single-cell screening in comparison to polyethylene glycol diacrylate (PEGDA).HeLa cells were encapsulated using a common polymerized pattern at 1000-2000 mJ/cm².The study revealed that 5% GelMA facilitated superior cell collection within two days due to enhanced profile retention.GelMA also demonstrated greater
Author: VENKATESH, KUMAR PANNEER SELVAM
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7035649959