Year: 2013
Type: article
Abstract: When treated with interferon-beta-1b, gene NCOA7 expresses a new variant that begins from an alternative start site NCAO7-AS with strong antioxidant properties. Segments of three different lengths of the upstream region of the gene were inserted into phRLnull. After testing and confirming their structure, the plasmids were transfected into mammalian cells. A luciferase assay was performed to determine which segments showed an increase in activity when treated with interferon. While each ligated plasmid acted as a strong promoter for Renilla luciferase, no significant difference in signal due to INF-beta- 1b treatment was observed.
Source: Digital WPI
Author: Koloski, Tyler James
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7039182614