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A new era in biological monitoring of toluene biomarker in urine through dansylation of o-cresol and LC-MS/MS analysis

Year: 2024
Type: article
Abstract: O-cresol in urine after hydrolysis is an exposure biomarker for toluene. This biomarker is volatile and routinely analyzed by gas chromatography−flame ionization detection (GC-FID) or gas chromatography−mass spectrometry (GC-MS). However, given the stringent sample preparation procedures required to ensure reliable GC-MS results, high-throughput sample analysis is not possible. In addition, the GC-FID detection limit for o-cresol in urine after hydrolysis is around 0.5 mg/L, while the ACGIH BEI is 0.3 mg/g cr (0.09 mg/L, if creatinine is 0.3 g/L; and 0.9 mg/L, if creatinine is 3 g/L). In other words, overexposure of some workers will not be detected by GC-FID as the detection limit is above the BEI set by ACGIH. Furthermore, GC-FID results are often inconclusive due to interference. This presentation, accordingly, describes a new alternative method that can measure as little as 10% of the ACGIH BEI and offer a throughput allowing results to be communicated to practitioners in less than 5 to 10 business days. O-cresol contain a functional group (hydroxyl group) that can react with dansyl chloride (dansylation reaction). This reaction allows conversion of the volatile o-cresol into non-volatile derivates compatible and enhanced with LC-MS/MS detection. The overall conditions established for this biomarker were validated based on AIHA LAP requirements for limits of detection and quantitation, intra-and inter-day precision, accuracy and recoveries. In addition, Clinchek QCs and interlaboratory proficiency tests from the G-EQUAS program were also performed. To our knowledge, our study is the first attempt to analyze o-cresol in urine after hydrolysis by LC-MS/MS.
Source: PhareSST
Authors: Gagné, Sébastien, Langlois, Eric
Language: English
Field: Chemistry
Open Access status: green
OpenAlex ID: W7039831787
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