Protective effect of CoCl2 on vancomycin-induced apoptosis of human renal tubular epithelial cells
Year: 2025
Type: article
Abstract: ObjectiveTo investigate the effect of CoCl2 on protecting vancomycin （VAN）-induced apoptosis of the human renal tubular epithelial cell line HK-2.MethodsHK-2 cells were cultured in vitro， and treated with blank control， 40 μg/mL VAN for 48 h， or pretreatment of 50 μmol/L CoCl2 for 6 h plus 40 μg/mL VAN for 48 h. Cell growth inhibition， and apoptotic rate were detected by cell counting kit-8 （CCK-8） assay and flow cytometry， respectively. Protein levels of HIF-1α and apoptosis-associated proteins （Caspase-3， Bcl-2） were detected by Western blot.ResultsCCK-8 assay showed that VAN dose-dependently inhibited the proliferation of HK-2 cells. Compared with blank control， VAN significantly induced apoptosis in HK-2 cells （P<0.05）. The apoptotic rate was significantly lower in CoCl2-pretreated HK-2 cells induced with VAN than those solely induced with VAN （P<0.05）. qRT-PCR and Western blot showed no significant differences in the mRNA and protein levels of HIF-1α between VAN-induced HK-2 cells and blank control （P>0.05）， which were significantly upregulated in CoCl2-pretreated HK-2 cells induced with VAN than those solely induced with VAN （P<0.05）. Caspase-3 was significantly upregulated， and Bcl-2 was significantly downregulated in VAN-induced HK-2 cells than blank control， which were significantly reversed by the pretreatment of CoCl2 （P<0.05）.ConclusionApoptosis is involved in VAN-induced HK-2 cells， and CoCl2 protects cellular damage in VAN-induced HK-2 cells by inhibiting cell apoptosis.
Authors: Liu Yuan, Yu Fen-fen, Feng Jing, Shan Wen-hong, Pan Pei-qiang, Zhang Hua, Ji Wen-xuan
Language: English
Topic: Acute Kidney Injury Research
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7042906687
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