Short-chain Fatty Acid-producing Faecalimonas Sp. Ngb245 Strain Regulates the Expression of Neuronal Activity-regulated Genes and Attenuates the Symptoms of Experimental Autoimmune Encephalomyelitis
Year: 2024
Type: article
Abstract: Alterations in gut microbiota and deregulation of the gut immune system are recognized as important events in autoimmune diseases. The knowledge about the important role of anaerobic gut bacteria that produce short-chain fatty acids (SCFAs), in the regulation of intestinal barrier and immune response made a way for the development of microbiota-based interventions. Our research aimed to isolate the strains with the potential to produce SCFAs, from healthy volunteer fecal material, and to test their effects on IL-8 production in the culture of intestinal epithelial cells (Caco2) as an in vitro system imitating initial intestinal inflammation, the effects on the expression of neuronal activity-regulated genes of Caenorhabditis elegans, and the effect on the development of experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis. Three isolated butyric acid (BA)-producing strains, and three acetic acid (AA)-producing strains diminished the production of IL-8 in Caco- 2 cells treated with IL-1β/TNF-α. Further, all BA-producing strains stimulated the expression of important neuro-related genes in C. elegans. Based on the strongest effects in these assays an isolate identified as Faecalimonas sp. NGB245 strain was further tested in EAE model. The oral treatment of EAE-induced mice with this strain for 16h per day for 15 days resulted in alleviated daily clinical scores, maximal clinical scores, and the duration of the illness in comparison to the effect of media used for strain cultivation. These results point to the potential of NGB245 to modify the gut-brain axis opening the field for future development of microbiota- based therapy for the diseases associated with immune response dysfunctions.
Authors: Bisenić, Aleksandar, Tomić, Sergej, Bekić, Marina, Pavlović, Luka, Dinić, Miroslav, Terzić- Vidojević, Amarela, Radojević, Dušan, Soković Bajić, Svetlana, Mitrović, Hristina, Jakovljević, Stefan, Stevanović, Dušan, Golić, Nataša, Đokić, Jelena
Language: English
Topic: Gut microbiota and health
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7043462725