Lactobacillus brevis-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins
Year: 2025
Type: article
Abstract: Yong-Han Cho,1 Ji-Woo Kim,1 Nari Kim,1 Hee-Sik Kim,1 Jun-Hwan Jang,1 Jun-Tae Bae,1 Wanil Kim2 1J2K-Metabiome, J2KBIO, Cheongju, Republic of Korea; 2Department of Biochemistry, Department of Convergence Medical Science, and Institute of Medical Science, Gyeongsang National University College of Medicine, Jinju, Republic of KoreaCorrespondence: Wanil Kim, Email [email protected]: The human skin, comprising the epidermis, dermis, and subcutaneous fat layers, serves as a critical barrier against external stimuli. The integrity of this barrier function is essential for preventing skin damage and diseases. When compromised, it can lead to various dermatological issues.Methods: This study investigated the efficacy of Lactobacillus brevis J2K55-derived exosomes (LBDEs) on enhancing skin barrier function. High-purity LBDEs were produced and characterized using nanoparticle tracking analysis and Cryo-TEM, concentrated to 1.52× 108 particles/mL with sizes ranging from 50 to 200 nm. The LBDEs were then applied to human keratinocytes, HaCaT cells, and a live human skin model to analyze the expression of genes significant to skin barrier function.Results: In vitro experiments demonstrated that 2.5% LBDEs increased Filaggrin mRNA expression by 301.80% compared to the control. In an ex vivo skin damage model induced by physical stimulation and UVB (Ultraviolet B) irradiation, 1% LBDEs treatment significantly upregulated the expression of key barrier-related proteins, including Aquaporin-3 (180.8%), Claudin-1 (205.4%), Filaggrin (309.9%), Loricrin (365.2%), and Serine palmitoyltransferase (191.3%), in comparison to the friction and UVB-induced control group.Conclusion: These results suggest that LBDEs have potential in enhancing skin barrier function, as evidenced by increased expression of crucial barrier-related proteins in both in vitro and ex vivo models. Keywords: exosome, skin barrier, lactobacillus brevis, nanoparticle tracking analysis, Cryo-TEM
Authors: Cho YH, Kim JW, Kim N, Kim HS, Jang JH, Bae JT, Kim W
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7048408289
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