Work

ChiraKit

Year: 2025

Type: article

Abstract: Circular dichroism (CD) spectroscopy is an established biophysical technique to study chiral molecules. CD allows investigating conformational changes under varying experimental conditions and has been used to understand secondary structure, folding, and binding of proteins and nucleic acids. Here, we present ChiraKit, a user-friendly, online, and open-source tool to process raw CD data and perform advanced analysis. ChiraKit features include the calculation of protein secondary structure with the SELCON3 and SESCA algorithms, estimation of peptide helicity using the helix-ensemble model, the fitting of thermal/chemical unfolding or user-defined models, and the decomposition of spectra through singular value decomposition or principal component analysis. ChiraKit can be accessed at https://spc.embl-hamburg.de/.

Source: Repository of the University of Ljubljana (University of Ljubljana)

Authors: Burastero, Osvaldo, Jones, Nykola C., Defelipe, Lucas A., Zavrtanik, Uroš, Hadži, San, Hoffmann, Søren Vrønning, Garcia-Alai, Maria

Institutions: European Molecular Biology Laboratory, European Molecular Biology Laboratory, Centre for Structural Systems Biology, Aarhus University, University of Ljubljana

Language: English

Topic: Protein Structure and Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Aarhus Universitet, Society for Research in Child Development, European Commission, H2020 Marie Skłodowska-Curie Actions

Awards: Career Accelerator for Research Infrastructure Scientists, MOlecular-Scale Biophysics Research Infrastructure, Career Accelerator for Research Infrastructure Scientists

OpenAlex ID: W7051209044

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