Year: 2024
Type: article
Abstract: Phosphoramidate benzoazole oligonucleotides (PABAO) are a newly developed oligonu- cleotides derivatives that carrying internucleotide N-(benzoazole)-phosphoramidate moieties. They can be obtained by the classical method of automated solid-phase phosphoramidate synthesis, in which the oxidation step is replaced by a Staudinger reaction with an azide modifier. In this work, optimization of the synthetic protocol of N-benzoxazole oligonucleotides was carried out. The optimization consisted of reducing the concentration of the azido reagent and shortening the synthesis time. With the adjustment, optimized synthesis conditions allow for a 2.5-fold decrease in benzoxazole azide consumption as well as a 20-min reduction in the time required for single modification introduction in oligonucleotide synthesis. The obtained advantages will be used for the scaled-up production of PABAOs in order to study them in biomedical applications
Authors: Baranoskaya, Elizaveta E., Poddubnyakova, Sofia A., Lomzov, Alexander A., Vasilyeva, Svetlana V., Барановская, Елизавета Е., Поддубнякова, Софья А., Ломзов, Александр А., Васильева, Светлана В.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7053522270
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