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Development and Characterization of Novel DNA Aptamers Specific to Heart-Type Fatty Acid Binding Protein (hFABP)

Year: 2024
Type: article
Abstract: Heart-type fatty acid-binding protein (hFABP) has been proposed as a new biochemical marker \nfor the early diagnosis of acute myocardial infarction (AMI). The work describes the development of \nhigh-affinity and specific DNA aptamers as sensor elements of analytical systems for the rapid detection \nof this marker. Several novel DNA aptamers to hFABP were selected by using hFABP-activated magnetic \nmicroparticles as a target. The DNA library enrichment, affinity and specificity of candidate aptamers \nas well their truncated variants, were examined by solid-phase obelin-based bioluminescent assay. High \nbinding ability was shown for the aptamer FABPAp4 by applying isothermal titration calorimetry (ITC) \ntechnique. The developed aptamers suggest to contain G-quadruplex (GQ) forming motifs that play a key role in binding the target. Demonstrated high affinity and specificity for hFABP determine the prospects \nof the obtained aptamers as sensor elements of analytical systems intended for AMI early diagnosis
Authors: Krasitskaya, Vasilisa V., Vereshchagina, Tatiana A., Smoliarova, Tatiana E., Sokolov, Alexey E., Tupikin, Alexey E., Dominguez-Arca, Vicente, Frank, Ludmila A., Красицкая, В. В., Верещагина, Т. А., Смолярова, Т. Е., Соколов, А. Э., Тупикин, А. Е., В. Домингес‑Арка, Франк, Л. А.
Institutions: Institute of Biophysics, Institute of Chemistry and Chemical Technology, Krasnoyarsk Scientific Center, Institute of Physics, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Universidade de Santiago de Compostela
Language: English
Open Access status: green
Award: G42242660
OpenAlex ID: W7056034686
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