Year: 2019
Type: article
Abstract: Retinoblastoma tumor suppressor-early-2 factor (Rb-E2F) pathway is pivotal in cell proliferation and tissue development.The E2F family is a group of transcription factors, which can be further divided into three subclasses: canonical activators (E2F1-3A/B), canonical repressors (E2F4-6) and atypical repressors (E2F7/8).E2F1-6 form heterodimer with dimerization partner (DP) proteins to bind DNA, while E2F7/8 do not bind with DP family but dimerize with each other or itself.The Rb family proteins repress E2F target gene expression by sequestering E2F1-3A/B activators and forming repressive protein complexes with E2F4/5.The atypical repressors, E2F7/8 repress gene expression independent of interaction with Rb family proteins.As a family of transcription factors, E2Fs regulate gene expression through directly binding to the consensus DNA sequence in promoter.E2F family has been identified as cell cycle regulator since 1990s, but how do different subclasses of E2Fs orchestrate to regulate cell cycle progression remains unclear.Recently, accumulating evidence indicates that E2Fs also play a role in differentiation to specify cell types in various tissues, but systematic analysis is missing in a physiological context.Therefore, the purpose of this study is to evaluate the expression of E2Fs in different cell cycle phase and determine differentiation role of E2Fs in an in vivo system.To approach the above purposes, we chose E2F3A, E2F4 and E2F8 (one from each subclass of E2F family) to investigate the orchestration of E2Fs during cell cycle in intact
Author: Sun, Daokun
Institution: The Ohio State University
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7056360815