Regulation of low density lipoprotein receptor related protein-1 expression by nerve growth factor in PC12 cells
Year: 2008
Type: dissertation
Abstract: LRP1 is an endocytic receptor involved in Alzheimer Disease (AD) pathology. Nerve growth factor, whose neuroprotective functions may be impaired in AD, modulates LRP1 expression. In this study, PC12 cells were treated with NGF and pharmacological inhibitors, in order to understand the regulation of LRP1 expression by NGF. Transfection of PC12 cells with LRP1 promoter constructs, and subsequent NGF treatments resulted in significant increases in LRP1 promoter activation. These were attributed to a putative response element located in the first kb of the LRP1 promoter. Inhibition of TrkA pathways showed possible involvement of all three pathways in LRP1 promoter activation. Inhibition of PKC activation resulted in the highest decrease in promoter activation. Additionally, inhibition of inducible nitric oxide synthase, both alone and with TrkA pathway inhibition, resulted in decreased promoter activation. Reverse transcription-PCR and immunoblotting did not show significant increases in LRP mRNA and protein, respectively, upon NGF treatment.
Author: Grana, Tomas R.
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7061796380