Characterization of exopolysaccharides from tempeh-associated lactic acid bacteria with anti-adhesion bioactivity towards enterotoxigenic Escherichia coli
Year: 2023
Type: conference-abstract
Abstract: Enterotoxigenic Escherichia coli (ETEC)-mediated diarrhea can be mitigated by inhibiting the adhesion of ETEC to the intestinal surface.Exopolysaccharides (EPS) from lactic acid bacteria (LAB) have been known to have anti-adhesion bioactivity towards ETEC.In this report, we aimed to characterize EPS from LAB isolated from tempeh-related environments for its structure and anti-adhesion bioactivity.Two strains of EPS-producing LAB were isolated from tempeh (Pediococcus pentosaceus strain TL and Leuconostoc mesenteroides strain TR) and soak water used in tempeh production (L.mesenteroides strains AW and NW).The EPS yields of TL and TR grown in liquid culture were 0.4% each, while AW and NW gave yields of 1.3% ± 0.4 and 1.1% ± 0.3, respectively.Carbohydrate analysis indicated that the EPS samples consisted of predominantly glucose and fructose, with the exception of TR, which consisted almost only of glucose.The EPS samples were degraded with carbohydrate-degrading enzymes for identification and released oligosaccharides were subjected to highperformance size exclusion chromatography.We found that TL, AW, and NW consisted of dextran (α-1,6 linked glucan with α-1,3 glucose branches; 1100 -1800 kDa) and levan (β-2,6 linked fructan with β-2,1 branches; 650 -760 kDa), while EPS from TR only consisted of dextran (828 kDa).EPS extracts from the four LAB isolates inhibited ETEC-mediated yeast agglutination and showed the capability to bind with ETEC.In conclusion, dextran and levan are major components of EPS produced by tempeh-associated LAB isolates and showed promising potential as an anti-adhesive agent towards ETEC.
Authors: Pramudito, T.E., Smid, E.J., Schols, H.A.
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7064366663