Year: 2019
Type: article
Abstract: During Clathrin Mediated Endocytosis (CME), Clathrin triskelia join to form a polyhedral cage on the interior of a cell's plasma membrane.As this cage is built, curvature of the membrane increases until a small piece is pinched off and internalized.This process serves a variety of important functions and is common and essential to all eukaryotic life.As such, it has been the most studied endocytic pathway since its discovery in the mid-1970s.Even so, fundamental questions remain about the mechanics of CME, which can vary wildly.This work seeks to explore the heterogeneities of clathrin coat structure and dynamics.The exact nature of the curvature-inducing forces has yet to be elucidated.But, no matter how the forces are generated, they will be directly opposed by any in-line tension held within the cell membrane.Our work has shown that CME dynamics are detectably altered by both spatial and temporal variations in membrane tension.Thus, endocytosis rates are strongly regulated by forces exerted on the membrane.As a result, observation of clathrin or clathrin coat components is sufficient to make meaningful commentary on changes in membrane tension.We are excited to propose analysis of clathrin dynamics as a convenient, widely accessible, and non-invasive method of probing membrane tension.Interest in membrane tension and mechano-sensation in general has been are a few who stand out as being critical to any amount of success I may have achieved by completing this document, and I would like to specially thank them here.First and foremost, my parents, who have always been supportive of my curiosity, even when my interests stray from the orthodox or practical.They are the type who gets their kid a microscope when he asks for one.For that I am always grateful.My high school physics teacher, Dr. Doug Skinner.He showed me that so many problems can be solved with math.I have never looked back.My colleagues, Joshua Ferguson, Scott Huber, and Comert Kural.This work is very much a collective effort.I would
Author: Willy, Nathan
Institution: The Ohio State University
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7066928892