Work

Methods to Evaluate Alterations in Polyamine Metabolism Caused by Helicobacter pylori Infection

Year: 2011

Type: book-chapter

Abstract: Helicobacter pylori is a Gram-negative bacteria that infects the human stomach of half of the world's -population. Colonization is followed by infiltration of the gastric mucosa by lymphocytes and myeloid cells. These cells are activated by various bacterial factors, causing them to produce immune/inflammatory mediators, including reactive nitrogen species and polyamines that contribute to cellular damage and the pathogenesis of H. pylori-associated gastric cancer. In vitro experiments have revealed that H. pylori induces macrophage polyamine production by upregulation of the arginase 2/ornithine decarboxylase (ODC) metabolic pathway and enhances hydrogen peroxide synthesis through the activity of spermidine oxidase (SMO). In this chapter, we present a survey of the methods used to analyze the induction and the role of the enzymes related to polyamine metabolism, i.e., arginase, ODC, and SMO in H. pylori-infected macrophages.

Source: Methods in molecular biology

DOI: 10.1007/978-1-61779-034-8_26

Authors: Alain P. Gobert, Rupesh Chaturvedi, Keith T. Wilson

Institutions: Institut National de la Recherche Agronomique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Vanderbilt University Medical Center, VA Tennessee Valley Healthcare System

Language: English

Topic: Polyamine Metabolism and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Complementary and Integrative Health