Mapping mammalian cell-type-specific transcriptional regulatory networks using KD-CAGE and ChIP-seq data in the TC-YIK cell line.
Type: dissertation
Abstract: Cell-type-specific TRNs by KD-CAGE and ChIP-seq Integration activates DACH1, SHOX2, PAX6, and PDX1.Analysis of these complementary datasets suggests the need for caution in interpreting ChIP-seq datasets.(1) A large fraction of binding sites are at distal enhancer sites and cannot be directly associated to their targets, without chromatin conformation data.(2) Many peaks may be non-functional: even when there is a peak at a promoter, the expression of the gene may not be affected in the matching perturbation experiment.
Author: Marina Lizio
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7073575640