Work

Heat-treated culture-dried Lactiplantibacillus Plantarum L-14 suppresses adipogenesis and lipogenesis in 3T3-L1 and HepG2 cells

Year: 2025

Type: article

Abstract: Obesity is a growing global health issue prompting the development of probiotic-based interventions. This study investigated whether heat-treated culture-dried L14 (HT-L14) retains these benefits. In HepG2 cells, HT-L14 reduced palmitate-induced lipid accumulation suppressed lipogenic genes including sterol regulatory element-binding protein 1 and carbohydrate-responsive element-binding protein, and activated AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), promoting lipid oxidation. In 3T3-L1 cells, HT-L14 inhibited adipocyte differentiation, reduced the expression of peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα), and increased energy metabolism-related gene expression. RNA sequencing analysis revealed differential gene expression enriched in lipid metabolism and insulin signaling pathways. HT-L14 exhibited no cytotoxicity and demonstrated improved safety compared to live L14 in HepG2 and 3T3-L1 cells. These findings suggest that HT-L14 retains anti-obesity efficacy with enhanced stability, supporting its potential as a therapeutic candidate for obesity and metabolic disorders.

Source: Journal of Functional Foods

DOI: 10.1016/j.jff.2025.106999