Gi50 Inhibition Study Using Sulforhodamine B (Srb) Assay Method On Mouse Melanoma Cell Line (B16-F10), Using Ethanolic Extracts Of Leaves Of Chromolaena Odorata And Capparis Moonii.
Year: 2025
Type: article
Abstract: The study investigates the in-vitro anticancer potential of ethanolic extracts from the leaves of Chromolaena odorata and Capparis moonii using the Sulforhodamine B (SRB) assay on the mouse melanoma cell line (B16-F10). These plants, rich in phytochemicals such as flavonoids and phenolic compounds, were hypothesized to possess significant anti-cancer properties. Extracts were prepared using Soxhlet apparatus and tested across multiple concentrations (10–80 μg/ml). Results demonstrated that the extract did not achieve the inhibitory concentration threshold (GI50 ≤ 20 μg/ml) defined by the National Cancer Institute for natural compounds. Morphological analysis and dose-response studies confirmed suboptimal inhibition of melanoma cell growth compared to the standard Adriamycin. While the extracts displayed a dose-dependent response, they were not effective in inhibiting melanoma growth. The findings suggest the need for enhanced experimental conditions and novel drug delivery systems to harness the therapeutic potential of these phytochemical-rich plants..
Source: Journal of Carcinogenesis
Authors: Urvashi Zamindar, Rajesh Khathuriya
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
OpenAlex ID: W7082647295
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