Work

The Dublin lens: a Cc = 1.0 mm objective lens intended for CryoEM at 100 keV

Year: 2025

Type: article

Abstract: We have designed, fabricated and tested a lens with a chromatic aberration coefficient (Cc) of 1.0 mm, a 4.0 mm pole-gap and 2.0 mm bore that is wide enough to accommodate an anti-contamination system and an objective aperture. This lens extends the temporal-coherence envelope of the electron microscope beyond 2 Å, using a low-cost Schottky FEG. We hope that this lens design can be used to improve all 100 keV electron microscopes designed for single-particle electron cryomicroscopy (cryoEM).

Source: Methods in microscopy

DOI: 10.1515/mim-2025-0019

Authors: Germano Motta Alves, Theo Andrews, Patrick McBean, Torquil Wells, Mohamed M. El-Gomati, Stephan Burkhalter, Clemens Schulze-Briese, Pietro Zambon, Greg McMullan, Richard Henderson, Christopher J. Russo, Lewys Jones

Institutions: Trinity College Dublin, DECTRIS (Switzerland), MRC Laboratory of Molecular Biology

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,260

Funders: AstraZeneca, Wellcome Trust, Astex Pharmaceuticals, Science Foundation Ireland, Irish Research Council, Akademie Věd České Republiky, Medical Research Council, Innovate UK

Awards: G1692867732, G1728860662, Affordable cryo-EM (FEG-TEM Upgrade), Electrifying Life Science, G617582727, Electrifying Life Science, AMBER_Phase 2

OpenAlex ID: W7082977299

FWCI: 1.026

Cites: 23

Cited by: 3

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