Isolation, screening, and characterization of hydrocarbon-degrading bacterial strains from soil contaminated with engine oil in Kut city
Year: 2024
Type: article
Abstract: Engine oil contamination is one of the foremost environmental problems caused by diverse human activities. Samples were collected from old garages and auto workshops and placed on a selective medium (Bushnell Haas mineral salt agar), with 1 % engine oil as the growth substrate. Thirty-one isolates from engine oil degraders were identified in eleven samples. Among these isolates, the samples (O9) 68 % and (O10) 64 % showed the highest degradation ability. Based on optical density measurement, biochemical testing, and molecular identification of the local isolate (O9); demonstrated that bacteria isolate (O9) belonging to Pseudomonas puttida, showed the highest biodegradation.
DOI: 10.31185/jwsm.568
Authors: Eman Falih Nimmah, Melad Khalaf Mohammed
Institution: University of Wasit
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W7082998165
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