Work

Analysis of gene expression changes in canola in response to inoculation with biotic and abiotic elicitors to Alternaria brassicae

Year: 2025

Type: article

Abstract: Background.Alternaria brassicaeis one of the most destructive pathogens of oilseed rape growth and production. Oilseed rape roots treated with biotic and abiotic elicitors have shown resistance to A. brassicaeas a result of induced systemic resistance (ISR). In this experiment, we aimed to identify resistance genes in two rapeseed genotypes (Raphanus brassicaand Brassica napus). Methods.Pseudomonas fluorescensPF83 as a biotic elicitor and with Salicylic acid (SA) as an abiotic elicitors were used induced systemic resistance in rapeseed. The expression levels of six defense-related genes (VSP2, MYC2, PR-2, PR-3, PR-4and PR-5) by Quantitative real-time PCR (qRT-PCR) at three times 24, 48 and 120 hpi. Results.VSP2, MYC2, PR-3and PR-4gene expression levels increased in infected leaves by A. brassicaeand in oilseed genotypes treated by PF83, while the expression levels of PR-2 and PR-5 were increased in leaves treated with SA. The results indicate that the induction of resistance genes depends on the type of elicitor, the biotic and abiotic elicitors interferes with phytohormones SA- and JA-pathways to A. brassicae, through the increase in the transcription of resistance-related genes. The abiotic elicitor is key to inducing resistance genes associated with SA-pathways, while the biotic elicitor is key to inducing resistance genes associated with JA-pathways. The results of our study provide a new understanding of the control mechanisms of A. brassica. Conclusion.This study provides a new understanding of the control mechanisms of A. brassica. The abiotic elicitor is key to inducing resistance genes associated with SA-pathways, while the biotic elicitor is key to inducing resistance genes associated with JA-pathways. This study explained, that the RR genome is responsible for disease resistance in oilseed R. brassica(AARR) to infection processes of A. brassicae, so we recommend that using R. brassica as resistance genotype to produce oilseed.

Source: Siberian Journal of Life Sciences and Agriculture

DOI: 10.12731/2658-6649-2025-17-2-1158

Author: Jawadayn Talib Alkooranee

Institution: University of Wasit

Language: English

Topic: Plant-Microbe Interactions and Immunity

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $350