Method Development and Validation for the Simultaneous Estimation of Ceftriaxone and Tazobactam in Bulk and Pharmaceutical Dosage Forms by Rp-Hplc
Year: 2025
Type: article
Abstract: A simple, accurate and precise High Performance Liquid Chromatographic (HPLC) method was developed and validated for the simultaneous estimation of Ceftriaxone and Tazobactam in bulk and Pharmaceutical dosage forms. The method was developed using Waters HPLC system with Inertsil – C18, ODS column (250 x 4.6 mm, 5µ). Mobile phase containing Methanol and buffer in the ratio 80:20 v/v in isocratic elution mode at a flow rate of 1.0ml/min (load of 20µl) . The detection was carried out at 254 nm. Retention time of Ceftriaxone and Tazobactam were found to be 3.049 min and 4.317 min respectively. The developed method was validated with respect to linearity, robustness, precision and accuracy. It was successfully applied for the simultaneous quantitative estimation of Ceftriaxone and Tazobactam in the Pharmaceutical dosage form. Results show that the retention and run time were decreased, so it is evident that the method developed was simple and economical that can be adopted in regular Quality control test in Industries
Authors: Dr. P. V. Madhavi Latha, Dr. Nagamani Bolla, Ms. K. Suvarna Ms. K. Suvarna, Dr. P. Uma Devi Dr. P. Uma Devi
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W7083463969
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