Work

Development of a Hybrid Composite Structure and Bioreactor for Enhanced Bone Regeneration in Dental Implants

Year: 2025

Type: article

Abstract: Dental implant surgery usually takes over 6 to 9 months, with 3 to 6 months specifically allocated for osseointegration between the implant and the surrounding bone. To expedite this process, we developed an innovative hybrid composite structure and a bioreactor. This hybrid structure features an assembly-type implant combined with a 3D-printed polycaprolactone (PCL) scaffold. The implant was redesigned in a modular format to enable the insertion of a scaffold between components, facilitating bone-to-bone contact instead of metal-to-bone contact, which enhances osseointegration. The PCL scaffold was coated with polydopamine (PDA) to improve cell adhesion. Additionally, a bioink that mimics bone composition, consisting of type I collagen and nano-hydroxyapatite (nHA), was incorporated into the scaffold. To support cell maturation within the scaffold, we developed a hydrostatic pressure bioreactor system that applies uniform compressive stress to complex 3D structures. We assessed cell viability in the scaffold using the CCK-8 assay, and SEM imaging confirmed the effectiveness of the PDA coating. Furthermore, we evaluated osteogenic differentiation through ALP activity and calcium quantification assays under both static and dynamic stimulation conditions.

Source: Journal of the Korean Society for Precision Engineering

DOI: 10.7736/jkspe.025.086

Authors: Eun Chae Kim, Jun-Kyu Kang, Hun-Jin Jeong, So-Jung Gwak, Seung-Jae Lee

Language: English

Topic: Bone Tissue Engineering Materials

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: Ministry of Trade, Industry and Energy, Institute of Civil-Military Technology Cooperation, Defense Acquisition Program Administration

Award: G1817251853